Integrated strategies of network pharmacology, transcriptomics, and computational and experimental validation reveal the anti-osteosarcoma effects of juglone via the ROS/PI3K/AKT pathway.

Summary: Osteosarcoma is a common and deadly type of bone cancer. Right now, patients need better treatments. Scientists studied a natural chemical called juglone, which is found in walnut trees, to see if it could help. They used computer models, lab tests on cells, and animal studies to figure out how it works. They discovered that juglone stops bone cancer cells from growing and spreading, and it even forces the cancer cells to die. It does this by blocking a specific chain of signals inside the cells (called the ROS/PI3K/AKT pathway). In animal tests, juglone safely shrank tumors over a short period. This means juglone could be a very promising new treatment for bone cancer in the future.